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p75 ntr flox mice  (Jackson Laboratory)

 
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    Jackson Laboratory p75 ntr flox mice
    Global <t>p75</t> <t>NTR</t> -deficient mice exhibited an osteoporotic phenotype. (A) Photos of 4 and 12-week-old p75 NTR+/+ and p75 NTR−/− mice. Scale bar: 4.0 cm. (B) Body weight curve of p75 NTR+/+ and p75 NTR−/− mice from P0 - P28. Three-dimensional Micro-CT images of 4 (C) and 12 (E) weeks old p75 NTR+/+ and p75 NTR−/− mice's whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th and length of femurs of 4 (D) and 12 (F) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 3) and p75 NTR−/− ( n = 4). Alcian blue staining images of the growth plate of 4 (G) and 12 (I) weeks old p75 NTR+/+ and p75 NTR−/− mice's tibias. The growth plate thickness (GP. Th) of 4 (H) and 12 (J) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 6) and p75 NTR−/− (n = 5). Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.
    P75 Ntr Flox Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p75 ntr flox mice/product/Jackson Laboratory
    Average 90 stars, based on 1 article reviews
    p75 ntr flox mice - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "p75 NTR regulates postnatal skeletal development via NGF-responsive JNK signaling"

    Article Title: p75 NTR regulates postnatal skeletal development via NGF-responsive JNK signaling

    Journal: Bone Reports

    doi: 10.1016/j.bonr.2025.101854

    Global p75 NTR -deficient mice exhibited an osteoporotic phenotype. (A) Photos of 4 and 12-week-old p75 NTR+/+ and p75 NTR−/− mice. Scale bar: 4.0 cm. (B) Body weight curve of p75 NTR+/+ and p75 NTR−/− mice from P0 - P28. Three-dimensional Micro-CT images of 4 (C) and 12 (E) weeks old p75 NTR+/+ and p75 NTR−/− mice's whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th and length of femurs of 4 (D) and 12 (F) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 3) and p75 NTR−/− ( n = 4). Alcian blue staining images of the growth plate of 4 (G) and 12 (I) weeks old p75 NTR+/+ and p75 NTR−/− mice's tibias. The growth plate thickness (GP. Th) of 4 (H) and 12 (J) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 6) and p75 NTR−/− (n = 5). Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.
    Figure Legend Snippet: Global p75 NTR -deficient mice exhibited an osteoporotic phenotype. (A) Photos of 4 and 12-week-old p75 NTR+/+ and p75 NTR−/− mice. Scale bar: 4.0 cm. (B) Body weight curve of p75 NTR+/+ and p75 NTR−/− mice from P0 - P28. Three-dimensional Micro-CT images of 4 (C) and 12 (E) weeks old p75 NTR+/+ and p75 NTR−/− mice's whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th and length of femurs of 4 (D) and 12 (F) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 3) and p75 NTR−/− ( n = 4). Alcian blue staining images of the growth plate of 4 (G) and 12 (I) weeks old p75 NTR+/+ and p75 NTR−/− mice's tibias. The growth plate thickness (GP. Th) of 4 (H) and 12 (J) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 6) and p75 NTR−/− (n = 5). Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.

    Techniques Used: Micro-CT, Staining

    MSC-specific p75 NTR -deficient mice exhibited an osteoporotic phenotype. (A) PCR results of p75 NTR in p75 NTRf/f and Prx1-Cre; p75 NTR f/f spleen, heart, liver, kidney, muscle, and bone. (B) PCR results of p75 NTR in p75 NTRf/f and Prx1-Cre; p75 NTR f/f monocyte and BMSC. p75 NTR expression was not detected in BMSCs. (C) Photos of 4 and 12-week-old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice. Scale bar: 4 cm. (D) Body weight of 4 and 12-week-old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice. For 4-week-old mice, p75 NTRf/f ( n = 3) and Prx1-Cre; p75 NTR f/f ( n = 4). For 12-week-old mice, p75 NTRf/f (n = 5) and Prx1-Cre; p75 NTR f/f (n = 4). Three-dimensional Micro-CT images of 4 (E) and 12 (G) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th, and length of femurs of 4 (F) and 12 (H) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, p75 NTRf/f ( n = 4) and Prx1-Cre; p75 NTR f/f ( n = 7). For 12-week-old mice, p75 NTRf/f (n = 6) and Prx1-Cre; p75 NTR f/f (n = 6). Alcian blue staining images of the growth plate of 4 (I) and 12 (K) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice's Tibias. The growth plate thickness (GP. Th) of 4 (J) and 12 (L) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice were quantified. For both ages, n = 5 for each group. Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.
    Figure Legend Snippet: MSC-specific p75 NTR -deficient mice exhibited an osteoporotic phenotype. (A) PCR results of p75 NTR in p75 NTRf/f and Prx1-Cre; p75 NTR f/f spleen, heart, liver, kidney, muscle, and bone. (B) PCR results of p75 NTR in p75 NTRf/f and Prx1-Cre; p75 NTR f/f monocyte and BMSC. p75 NTR expression was not detected in BMSCs. (C) Photos of 4 and 12-week-old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice. Scale bar: 4 cm. (D) Body weight of 4 and 12-week-old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice. For 4-week-old mice, p75 NTRf/f ( n = 3) and Prx1-Cre; p75 NTR f/f ( n = 4). For 12-week-old mice, p75 NTRf/f (n = 5) and Prx1-Cre; p75 NTR f/f (n = 4). Three-dimensional Micro-CT images of 4 (E) and 12 (G) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th, and length of femurs of 4 (F) and 12 (H) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, p75 NTRf/f ( n = 4) and Prx1-Cre; p75 NTR f/f ( n = 7). For 12-week-old mice, p75 NTRf/f (n = 6) and Prx1-Cre; p75 NTR f/f (n = 6). Alcian blue staining images of the growth plate of 4 (I) and 12 (K) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice's Tibias. The growth plate thickness (GP. Th) of 4 (J) and 12 (L) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice were quantified. For both ages, n = 5 for each group. Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.

    Techniques Used: Expressing, Micro-CT, Staining

    p75 NTR mediates osteogenic differentiation of MSCs. (A) Schematic representation of the osteogenic differentiation process of BMSCs derived from p75 NTR+/+ and p75 NTR−/− mice. Created in BioRender. Kadota, C. (2025) https://BioRender.com/a77c926 (B) Alizarin Red Staining (ARS) images of BMSCs harvested from p75 NTR+/+ and p75 NTR−/− mice cultured in GM and OIM for 21 days, along with quantification of Alizarin Red-positive areas. n = 4 for each group. (C) qRT-PCR analysis of osteoblast differentiation markers in BMSCs cultured in OIM. The expression of osteoblastic differentiation markers, including PTHrP , Runx2 , Dlx5 , Osx , and Alp , were compared, and p75 NTR−/− showed significant downregulation compared to p75 NTR+/+ . n = 3 for each group. Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of experimental replicates.
    Figure Legend Snippet: p75 NTR mediates osteogenic differentiation of MSCs. (A) Schematic representation of the osteogenic differentiation process of BMSCs derived from p75 NTR+/+ and p75 NTR−/− mice. Created in BioRender. Kadota, C. (2025) https://BioRender.com/a77c926 (B) Alizarin Red Staining (ARS) images of BMSCs harvested from p75 NTR+/+ and p75 NTR−/− mice cultured in GM and OIM for 21 days, along with quantification of Alizarin Red-positive areas. n = 4 for each group. (C) qRT-PCR analysis of osteoblast differentiation markers in BMSCs cultured in OIM. The expression of osteoblastic differentiation markers, including PTHrP , Runx2 , Dlx5 , Osx , and Alp , were compared, and p75 NTR−/− showed significant downregulation compared to p75 NTR+/+ . n = 3 for each group. Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of experimental replicates.

    Techniques Used: Derivative Assay, Staining, Cell Culture, Quantitative RT-PCR, Expressing

    p75 NTR -deficient BMSCs showed reduced NGF signaling and MAPK activation. (A) Volcano plot shows gene expression changes in bone marrow stem cells (BMSCs) derived from 4-week-old p75 NTR+/+ and p75 NTR−/− mice (n = 3 per group). Expression was profiled using the NanoString nCounter platform in a panel of 477 genes. The x-axis represents the log 2 fold change in p75 NTR−/− relative to p75 NTR+/+ and the y-axis is the –log 10 ( p -value). The dashed horizontal line indicates p = 0.05. Among the 637 genes tested, 15 were significantly downregulated (p < 0.05) in p75 NTR−/− BMSCs. Red dots indicate upregulated genes, while blue dots indicate downregulated genes in p75 NTR−/− . (B) Heatmap of the 15 differentially expressed genes. Red indicates higher expression, while blue indicates lower expression. (C)-(E) Bar graph of pathway enrichment analysis for the 15 differentially expressed genes. The analysis was performed using the BioPlanet 2019, (C) BioCarta 2016, and (E) Reactome pathways 2024. The y-axis lists the top 15 significantly enriched terms (p < 0.05), and the x-axis shows the –log 10 (p-value). n represents the number of experimental replicates.
    Figure Legend Snippet: p75 NTR -deficient BMSCs showed reduced NGF signaling and MAPK activation. (A) Volcano plot shows gene expression changes in bone marrow stem cells (BMSCs) derived from 4-week-old p75 NTR+/+ and p75 NTR−/− mice (n = 3 per group). Expression was profiled using the NanoString nCounter platform in a panel of 477 genes. The x-axis represents the log 2 fold change in p75 NTR−/− relative to p75 NTR+/+ and the y-axis is the –log 10 ( p -value). The dashed horizontal line indicates p = 0.05. Among the 637 genes tested, 15 were significantly downregulated (p < 0.05) in p75 NTR−/− BMSCs. Red dots indicate upregulated genes, while blue dots indicate downregulated genes in p75 NTR−/− . (B) Heatmap of the 15 differentially expressed genes. Red indicates higher expression, while blue indicates lower expression. (C)-(E) Bar graph of pathway enrichment analysis for the 15 differentially expressed genes. The analysis was performed using the BioPlanet 2019, (C) BioCarta 2016, and (E) Reactome pathways 2024. The y-axis lists the top 15 significantly enriched terms (p < 0.05), and the x-axis shows the –log 10 (p-value). n represents the number of experimental replicates.

    Techniques Used: Activation Assay, Gene Expression, Derivative Assay, Expressing

    KDM4B regulates p75 NTR -mediated osteogenesis via JNK signaling pathway. (A)Western blot of JNK signaling pathway (p-JNK and p-c-Jun) in p75 NTR+/+ and p75 NTR−/− BMSCs. (B) ARS images and quantification after 21 days treatment in OIM with NGF (10 ng/ml) and/or SP600125 (5 μM). n = 4 for each group. (C) qRT-PCR analysis of epigenetic genes in p75 NTR+/+ and p75 NTR−/− BMSCs with or without NGF treatment at 2 h. n = 3 for each group. (D) ARS images and quantification after 21 days treatment in OIM with pLV-empty or pLV- Kdm4b , and NGF (10 ng/ml). n = 4 for each group. (E) Upper panel: ARS images and quantification after 21 days treatment in OIM with pLV-empty or pLV- Kdm4b in p75 NTR+/+ and p75 NTR−/− BMSCs. Lower panel: gene expression analysis of Kdm4b and Dlx5 after treating with pLV-empty or pLV- Kdm4b . Dlx5 expression increased along with the upregulation of Kdm4b . n = 4 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001. Two-way ANOVA with multiple comparison was used for c, d and e, followed by Tukey's post-hoc test. n represents the number of experimental replicates.
    Figure Legend Snippet: KDM4B regulates p75 NTR -mediated osteogenesis via JNK signaling pathway. (A)Western blot of JNK signaling pathway (p-JNK and p-c-Jun) in p75 NTR+/+ and p75 NTR−/− BMSCs. (B) ARS images and quantification after 21 days treatment in OIM with NGF (10 ng/ml) and/or SP600125 (5 μM). n = 4 for each group. (C) qRT-PCR analysis of epigenetic genes in p75 NTR+/+ and p75 NTR−/− BMSCs with or without NGF treatment at 2 h. n = 3 for each group. (D) ARS images and quantification after 21 days treatment in OIM with pLV-empty or pLV- Kdm4b , and NGF (10 ng/ml). n = 4 for each group. (E) Upper panel: ARS images and quantification after 21 days treatment in OIM with pLV-empty or pLV- Kdm4b in p75 NTR+/+ and p75 NTR−/− BMSCs. Lower panel: gene expression analysis of Kdm4b and Dlx5 after treating with pLV-empty or pLV- Kdm4b . Dlx5 expression increased along with the upregulation of Kdm4b . n = 4 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001. Two-way ANOVA with multiple comparison was used for c, d and e, followed by Tukey's post-hoc test. n represents the number of experimental replicates.

    Techniques Used: Western Blot, Quantitative RT-PCR, Gene Expression, Expressing, Comparison



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    p75 ntr flox mice  (Jackson Laboratory)
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    Jackson Laboratory p75 ntr flox mice
    Global <t>p75</t> <t>NTR</t> -deficient mice exhibited an osteoporotic phenotype. (A) Photos of 4 and 12-week-old p75 NTR+/+ and p75 NTR−/− mice. Scale bar: 4.0 cm. (B) Body weight curve of p75 NTR+/+ and p75 NTR−/− mice from P0 - P28. Three-dimensional Micro-CT images of 4 (C) and 12 (E) weeks old p75 NTR+/+ and p75 NTR−/− mice's whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th and length of femurs of 4 (D) and 12 (F) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 3) and p75 NTR−/− ( n = 4). Alcian blue staining images of the growth plate of 4 (G) and 12 (I) weeks old p75 NTR+/+ and p75 NTR−/− mice's tibias. The growth plate thickness (GP. Th) of 4 (H) and 12 (J) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 6) and p75 NTR−/− (n = 5). Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.
    P75 Ntr Flox Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p75 ntr flox mice/product/Jackson Laboratory
    Average 90 stars, based on 1 article reviews
    p75 ntr flox mice - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

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    Global p75 NTR -deficient mice exhibited an osteoporotic phenotype. (A) Photos of 4 and 12-week-old p75 NTR+/+ and p75 NTR−/− mice. Scale bar: 4.0 cm. (B) Body weight curve of p75 NTR+/+ and p75 NTR−/− mice from P0 - P28. Three-dimensional Micro-CT images of 4 (C) and 12 (E) weeks old p75 NTR+/+ and p75 NTR−/− mice's whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th and length of femurs of 4 (D) and 12 (F) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 3) and p75 NTR−/− ( n = 4). Alcian blue staining images of the growth plate of 4 (G) and 12 (I) weeks old p75 NTR+/+ and p75 NTR−/− mice's tibias. The growth plate thickness (GP. Th) of 4 (H) and 12 (J) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 6) and p75 NTR−/− (n = 5). Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.

    Journal: Bone Reports

    Article Title: p75 NTR regulates postnatal skeletal development via NGF-responsive JNK signaling

    doi: 10.1016/j.bonr.2025.101854

    Figure Lengend Snippet: Global p75 NTR -deficient mice exhibited an osteoporotic phenotype. (A) Photos of 4 and 12-week-old p75 NTR+/+ and p75 NTR−/− mice. Scale bar: 4.0 cm. (B) Body weight curve of p75 NTR+/+ and p75 NTR−/− mice from P0 - P28. Three-dimensional Micro-CT images of 4 (C) and 12 (E) weeks old p75 NTR+/+ and p75 NTR−/− mice's whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th and length of femurs of 4 (D) and 12 (F) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 3) and p75 NTR−/− ( n = 4). Alcian blue staining images of the growth plate of 4 (G) and 12 (I) weeks old p75 NTR+/+ and p75 NTR−/− mice's tibias. The growth plate thickness (GP. Th) of 4 (H) and 12 (J) weeks old p75 NTR+/+ and p75 NTR− /− mice were quantified. For 4-week-old mice, n = 5 for each group. For 12-week-old mice, p75 NTR+/+ ( n = 6) and p75 NTR−/− (n = 5). Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.

    Article Snippet: p75 NTR−/− (Stock No: 002213), p75 NTR flox (p75 NTRf/f ) (Stock No: 031162), and paired related homeobox-Cre (Prx1-Cre) (Stock No: 005584) mice were obtained from the Jackson Laboratory (Bar Harbor, ME) and housed in the animal facility at the University of California, San Francisco (UCSF) according to institutional guidelines.

    Techniques: Micro-CT, Staining

    MSC-specific p75 NTR -deficient mice exhibited an osteoporotic phenotype. (A) PCR results of p75 NTR in p75 NTRf/f and Prx1-Cre; p75 NTR f/f spleen, heart, liver, kidney, muscle, and bone. (B) PCR results of p75 NTR in p75 NTRf/f and Prx1-Cre; p75 NTR f/f monocyte and BMSC. p75 NTR expression was not detected in BMSCs. (C) Photos of 4 and 12-week-old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice. Scale bar: 4 cm. (D) Body weight of 4 and 12-week-old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice. For 4-week-old mice, p75 NTRf/f ( n = 3) and Prx1-Cre; p75 NTR f/f ( n = 4). For 12-week-old mice, p75 NTRf/f (n = 5) and Prx1-Cre; p75 NTR f/f (n = 4). Three-dimensional Micro-CT images of 4 (E) and 12 (G) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th, and length of femurs of 4 (F) and 12 (H) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, p75 NTRf/f ( n = 4) and Prx1-Cre; p75 NTR f/f ( n = 7). For 12-week-old mice, p75 NTRf/f (n = 6) and Prx1-Cre; p75 NTR f/f (n = 6). Alcian blue staining images of the growth plate of 4 (I) and 12 (K) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice's Tibias. The growth plate thickness (GP. Th) of 4 (J) and 12 (L) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice were quantified. For both ages, n = 5 for each group. Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.

    Journal: Bone Reports

    Article Title: p75 NTR regulates postnatal skeletal development via NGF-responsive JNK signaling

    doi: 10.1016/j.bonr.2025.101854

    Figure Lengend Snippet: MSC-specific p75 NTR -deficient mice exhibited an osteoporotic phenotype. (A) PCR results of p75 NTR in p75 NTRf/f and Prx1-Cre; p75 NTR f/f spleen, heart, liver, kidney, muscle, and bone. (B) PCR results of p75 NTR in p75 NTRf/f and Prx1-Cre; p75 NTR f/f monocyte and BMSC. p75 NTR expression was not detected in BMSCs. (C) Photos of 4 and 12-week-old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice. Scale bar: 4 cm. (D) Body weight of 4 and 12-week-old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice. For 4-week-old mice, p75 NTRf/f ( n = 3) and Prx1-Cre; p75 NTR f/f ( n = 4). For 12-week-old mice, p75 NTRf/f (n = 5) and Prx1-Cre; p75 NTR f/f (n = 4). Three-dimensional Micro-CT images of 4 (E) and 12 (G) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice whole femurs, trabecular bones, and cortical bones. The BV/TV, Tb. Th, Tb.N, CtV/TV, Ct. Th, and length of femurs of 4 (F) and 12 (H) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice were quantified by 3D Micro-CT. Scale bar (left panel): 1 mm, Scale bar (right panel): 0.5 mm. For 4-week-old mice, p75 NTRf/f ( n = 4) and Prx1-Cre; p75 NTR f/f ( n = 7). For 12-week-old mice, p75 NTRf/f (n = 6) and Prx1-Cre; p75 NTR f/f (n = 6). Alcian blue staining images of the growth plate of 4 (I) and 12 (K) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice's Tibias. The growth plate thickness (GP. Th) of 4 (J) and 12 (L) weeks old p75 NTRf/f and Prx1-Cre; p75 NTR f/f mice were quantified. For both ages, n = 5 for each group. Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of mice analyzed.

    Article Snippet: p75 NTR−/− (Stock No: 002213), p75 NTR flox (p75 NTRf/f ) (Stock No: 031162), and paired related homeobox-Cre (Prx1-Cre) (Stock No: 005584) mice were obtained from the Jackson Laboratory (Bar Harbor, ME) and housed in the animal facility at the University of California, San Francisco (UCSF) according to institutional guidelines.

    Techniques: Expressing, Micro-CT, Staining

    p75 NTR mediates osteogenic differentiation of MSCs. (A) Schematic representation of the osteogenic differentiation process of BMSCs derived from p75 NTR+/+ and p75 NTR−/− mice. Created in BioRender. Kadota, C. (2025) https://BioRender.com/a77c926 (B) Alizarin Red Staining (ARS) images of BMSCs harvested from p75 NTR+/+ and p75 NTR−/− mice cultured in GM and OIM for 21 days, along with quantification of Alizarin Red-positive areas. n = 4 for each group. (C) qRT-PCR analysis of osteoblast differentiation markers in BMSCs cultured in OIM. The expression of osteoblastic differentiation markers, including PTHrP , Runx2 , Dlx5 , Osx , and Alp , were compared, and p75 NTR−/− showed significant downregulation compared to p75 NTR+/+ . n = 3 for each group. Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of experimental replicates.

    Journal: Bone Reports

    Article Title: p75 NTR regulates postnatal skeletal development via NGF-responsive JNK signaling

    doi: 10.1016/j.bonr.2025.101854

    Figure Lengend Snippet: p75 NTR mediates osteogenic differentiation of MSCs. (A) Schematic representation of the osteogenic differentiation process of BMSCs derived from p75 NTR+/+ and p75 NTR−/− mice. Created in BioRender. Kadota, C. (2025) https://BioRender.com/a77c926 (B) Alizarin Red Staining (ARS) images of BMSCs harvested from p75 NTR+/+ and p75 NTR−/− mice cultured in GM and OIM for 21 days, along with quantification of Alizarin Red-positive areas. n = 4 for each group. (C) qRT-PCR analysis of osteoblast differentiation markers in BMSCs cultured in OIM. The expression of osteoblastic differentiation markers, including PTHrP , Runx2 , Dlx5 , Osx , and Alp , were compared, and p75 NTR−/− showed significant downregulation compared to p75 NTR+/+ . n = 3 for each group. Unpaired Student's t -test was used for analysis, with * p < 0.05, ** p < 0.01, *** p < 0.001. n represents the number of experimental replicates.

    Article Snippet: p75 NTR−/− (Stock No: 002213), p75 NTR flox (p75 NTRf/f ) (Stock No: 031162), and paired related homeobox-Cre (Prx1-Cre) (Stock No: 005584) mice were obtained from the Jackson Laboratory (Bar Harbor, ME) and housed in the animal facility at the University of California, San Francisco (UCSF) according to institutional guidelines.

    Techniques: Derivative Assay, Staining, Cell Culture, Quantitative RT-PCR, Expressing

    p75 NTR -deficient BMSCs showed reduced NGF signaling and MAPK activation. (A) Volcano plot shows gene expression changes in bone marrow stem cells (BMSCs) derived from 4-week-old p75 NTR+/+ and p75 NTR−/− mice (n = 3 per group). Expression was profiled using the NanoString nCounter platform in a panel of 477 genes. The x-axis represents the log 2 fold change in p75 NTR−/− relative to p75 NTR+/+ and the y-axis is the –log 10 ( p -value). The dashed horizontal line indicates p = 0.05. Among the 637 genes tested, 15 were significantly downregulated (p < 0.05) in p75 NTR−/− BMSCs. Red dots indicate upregulated genes, while blue dots indicate downregulated genes in p75 NTR−/− . (B) Heatmap of the 15 differentially expressed genes. Red indicates higher expression, while blue indicates lower expression. (C)-(E) Bar graph of pathway enrichment analysis for the 15 differentially expressed genes. The analysis was performed using the BioPlanet 2019, (C) BioCarta 2016, and (E) Reactome pathways 2024. The y-axis lists the top 15 significantly enriched terms (p < 0.05), and the x-axis shows the –log 10 (p-value). n represents the number of experimental replicates.

    Journal: Bone Reports

    Article Title: p75 NTR regulates postnatal skeletal development via NGF-responsive JNK signaling

    doi: 10.1016/j.bonr.2025.101854

    Figure Lengend Snippet: p75 NTR -deficient BMSCs showed reduced NGF signaling and MAPK activation. (A) Volcano plot shows gene expression changes in bone marrow stem cells (BMSCs) derived from 4-week-old p75 NTR+/+ and p75 NTR−/− mice (n = 3 per group). Expression was profiled using the NanoString nCounter platform in a panel of 477 genes. The x-axis represents the log 2 fold change in p75 NTR−/− relative to p75 NTR+/+ and the y-axis is the –log 10 ( p -value). The dashed horizontal line indicates p = 0.05. Among the 637 genes tested, 15 were significantly downregulated (p < 0.05) in p75 NTR−/− BMSCs. Red dots indicate upregulated genes, while blue dots indicate downregulated genes in p75 NTR−/− . (B) Heatmap of the 15 differentially expressed genes. Red indicates higher expression, while blue indicates lower expression. (C)-(E) Bar graph of pathway enrichment analysis for the 15 differentially expressed genes. The analysis was performed using the BioPlanet 2019, (C) BioCarta 2016, and (E) Reactome pathways 2024. The y-axis lists the top 15 significantly enriched terms (p < 0.05), and the x-axis shows the –log 10 (p-value). n represents the number of experimental replicates.

    Article Snippet: p75 NTR−/− (Stock No: 002213), p75 NTR flox (p75 NTRf/f ) (Stock No: 031162), and paired related homeobox-Cre (Prx1-Cre) (Stock No: 005584) mice were obtained from the Jackson Laboratory (Bar Harbor, ME) and housed in the animal facility at the University of California, San Francisco (UCSF) according to institutional guidelines.

    Techniques: Activation Assay, Gene Expression, Derivative Assay, Expressing

    KDM4B regulates p75 NTR -mediated osteogenesis via JNK signaling pathway. (A)Western blot of JNK signaling pathway (p-JNK and p-c-Jun) in p75 NTR+/+ and p75 NTR−/− BMSCs. (B) ARS images and quantification after 21 days treatment in OIM with NGF (10 ng/ml) and/or SP600125 (5 μM). n = 4 for each group. (C) qRT-PCR analysis of epigenetic genes in p75 NTR+/+ and p75 NTR−/− BMSCs with or without NGF treatment at 2 h. n = 3 for each group. (D) ARS images and quantification after 21 days treatment in OIM with pLV-empty or pLV- Kdm4b , and NGF (10 ng/ml). n = 4 for each group. (E) Upper panel: ARS images and quantification after 21 days treatment in OIM with pLV-empty or pLV- Kdm4b in p75 NTR+/+ and p75 NTR−/− BMSCs. Lower panel: gene expression analysis of Kdm4b and Dlx5 after treating with pLV-empty or pLV- Kdm4b . Dlx5 expression increased along with the upregulation of Kdm4b . n = 4 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001. Two-way ANOVA with multiple comparison was used for c, d and e, followed by Tukey's post-hoc test. n represents the number of experimental replicates.

    Journal: Bone Reports

    Article Title: p75 NTR regulates postnatal skeletal development via NGF-responsive JNK signaling

    doi: 10.1016/j.bonr.2025.101854

    Figure Lengend Snippet: KDM4B regulates p75 NTR -mediated osteogenesis via JNK signaling pathway. (A)Western blot of JNK signaling pathway (p-JNK and p-c-Jun) in p75 NTR+/+ and p75 NTR−/− BMSCs. (B) ARS images and quantification after 21 days treatment in OIM with NGF (10 ng/ml) and/or SP600125 (5 μM). n = 4 for each group. (C) qRT-PCR analysis of epigenetic genes in p75 NTR+/+ and p75 NTR−/− BMSCs with or without NGF treatment at 2 h. n = 3 for each group. (D) ARS images and quantification after 21 days treatment in OIM with pLV-empty or pLV- Kdm4b , and NGF (10 ng/ml). n = 4 for each group. (E) Upper panel: ARS images and quantification after 21 days treatment in OIM with pLV-empty or pLV- Kdm4b in p75 NTR+/+ and p75 NTR−/− BMSCs. Lower panel: gene expression analysis of Kdm4b and Dlx5 after treating with pLV-empty or pLV- Kdm4b . Dlx5 expression increased along with the upregulation of Kdm4b . n = 4 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001. Two-way ANOVA with multiple comparison was used for c, d and e, followed by Tukey's post-hoc test. n represents the number of experimental replicates.

    Article Snippet: p75 NTR−/− (Stock No: 002213), p75 NTR flox (p75 NTRf/f ) (Stock No: 031162), and paired related homeobox-Cre (Prx1-Cre) (Stock No: 005584) mice were obtained from the Jackson Laboratory (Bar Harbor, ME) and housed in the animal facility at the University of California, San Francisco (UCSF) according to institutional guidelines.

    Techniques: Western Blot, Quantitative RT-PCR, Gene Expression, Expressing, Comparison